Hotspot

trnQ-UUG-accD

Genome · 54268-54653 · 386 bp

Back to Hedysarum

Pi 0.0697

MarkerSeek score 79.20

Primer available yes

Label trnQ-UUG-accD

Feature Metadata

label_name: trnQ-UUG-accD
feature_type: igs
region: Genome
length_bp: 386
variable_sites: 196
pi: 0.0697
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-accD_p1	GAGGTTCGAATCCTTCCGTC	TCACCAACTACGGATCCCAT	55.0	50.0	58.1	58.1	1073-2109 bp	1.0	90.4
trnQ-UUG-accD_p2	GAGGTTCGAATCCTTCCGTC	TCTTCGCATTGAACCCACAA	55.0	45.0	58.1	58.0	737-1195 bp	0.92	88.0
trnQ-UUG-accD_p3	GAGGTTCGAATCCTTCCGTC	CTAGTACCCGTGCTTTCACC	55.0	55.0	58.1	58.0	589-903 bp	0.84	81.9
trnQ-UUG-accD_p4	AGATCAGACCTCTTTCCCCC	CTAGTACCCGTGCTTTCACC	55.0	55.0	58.1	58.0	902-1226 bp	0.84	81.1
trnQ-UUG-accD_p5	AGATCAGACCTCTTTCCCCC	TCTTCGCATTGAACCCACAA	55.0	45.0	58.1	58.0	1068-1328 bp	0.84	81.0