Hotspot

trnY-GUA-trnD-GUC

Genome · 30214-30686 · 473 bp

Back to Hedysarum

Pi 0.037

MarkerSeek score 82.80

Primer available yes

Label trnY-GUA-trnD-GUC

Feature Metadata

label_name: trnY-GUA-trnD-GUC
feature_type: igs
region: Genome
length_bp: 473
variable_sites: 92
pi: 0.037
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-GUA-trnD-GUC_p1	TGGCAATATGTCTACGCTGG	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.8	58.5	499-580 bp	1.0	86.3
trnY-GUA-trnD-GUC_p2	GGCAATATGTCTACGCTGGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.8	58.5	498-579 bp	1.0	86.3
trnY-GUA-trnD-GUC_p3	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	520-601 bp	1.0	85.4
trnY-GUA-trnD-GUC_p4	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.6	58.5	519-600 bp	1.0	85.2
trnY-GUA-trnD-GUC_p5	GATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	55.0	50.0	58.4	58.5	553-634 bp	1.0	84.9