Hotspot

trnK-UUU-rbcL

Genome · 4178-5070 · 893 bp

Back to Hedysarum

Pi 0.0265

MarkerSeek score 80.20

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: Genome
length_bp: 893
variable_sites: 133
pi: 0.0265
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	TGGAGTCGCACTTAAAAGCC	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.5	58.0	1012-1198 bp	1.0	87.9
trnK-UUU-rbcL_p2	AACCCTACCGATGGTATGGA	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.5	58.0	1065-1251 bp	1.0	87.9
trnK-UUU-rbcL_p3	AGTCGTGGTCTTACAAACCC	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.5	58.0	1080-1266 bp	1.0	87.4
trnK-UUU-rbcL_p4	GTCGTGGTCTTACAAACCCT	AGTCCTGAATTAGCTGCTGC	50.0	50.0	57.5	58.0	1079-1265 bp	1.0	87.4
trnK-UUU-rbcL_p5	AGTCGCACTTAAAAGCCGAG	AGTCCTGAATTAGCTGCTGC	50.0	50.0	58.6	58.0	1009-1195 bp	1.0	87.2