primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	TCGCGTCTCTCTAAAATTGCA	TGCAATGAAAAATGCACGCA	21	20	42.9	40.0	58.0	58.1	5.8	0.0	1.183713	1370	1703	434-534	1.000000	82.5
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	TCGCGTCTCTCTAAAATTGCA	GCAATGAAAAATGCACGCAC	21	20	42.9	45.0	58.0	57.8	5.8	0.0	1.261558	1370	1703	433-533	1.000000	81.9
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	CGCGTCTCTCTAAAATTGCAG	TGCAATGAAAAATGCACGCA	21	20	47.6	40.0	57.7	58.1	0.0	0.0	1.433582	1370	1703	433-533	1.000000	80.5
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	CGCGTCTCTCTAAAATTGCAG	GCAATGAAAAATGCACGCAC	21	20	47.6	45.0	57.7	57.8	0.0	0.0	1.511427	1370	1703	432-532	1.000000	79.9
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	TCGCGTCTCTCTAAAATTGCA	CAATGAAAAATGCACGCACG	21	20	42.9	45.0	58.0	57.5	5.8	0.0	1.520006	1370	1703	432-532	1.000000	79.8
trnK-UUU-rbcL_p1	trnK-UUU-rbcL	1	TGGAGTCGCACTTAAAAGCC	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	58.5	58.0	0.0	13.4	0.516122	4178	5070	1012-1198	1.000000	87.9
trnK-UUU-rbcL_p2	trnK-UUU-rbcL	2	AACCCTACCGATGGTATGGA	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	57.5	58.0	20.0	13.4	0.517944	4178	5070	1065-1251	1.000000	87.9
trnK-UUU-rbcL_p3	trnK-UUU-rbcL	3	AGTCGTGGTCTTACAAACCC	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	57.5	58.0	0.0	13.4	0.574042	4178	5070	1080-1266	1.000000	87.4
trnK-UUU-rbcL_p4	trnK-UUU-rbcL	4	GTCGTGGTCTTACAAACCCT	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	57.5	58.0	0.0	13.4	0.574042	4178	5070	1079-1265	1.000000	87.4
trnK-UUU-rbcL_p5	trnK-UUU-rbcL	5	AGTCGCACTTAAAAGCCGAG	AGTCCTGAATTAGCTGCTGC	20	20	50.0	50.0	58.6	58.0	6.4	13.4	0.606067	4178	5070	1009-1195	1.000000	87.2
trnG-GCC-psbZ_p1	trnG-GCC-psbZ	1	CGGATAGCGGGAATCGAAC	CTTCTCCTGATGGTTGGTCG	19	20	57.9	55.0	58.2	58.0	0.0	0.0	1.205250	24480	25381	531-1087	1.000000	82.4
trnG-GCC-psbZ_p2	trnG-GCC-psbZ	2	GATAGCGGGAATCGAACCC	CTTCTCCTGATGGTTGGTCG	19	20	57.9	55.0	57.8	58.0	17.7	0.0	1.228186	24480	25381	529-1085	1.000000	82.2
trnG-GCC-psbZ_p3	trnG-GCC-psbZ	3	GGATAGCGGGAATCGAACC	CTTCTCCTGATGGTTGGTCG	19	20	57.9	55.0	57.8	58.0	0.0	0.0	1.228186	24480	25381	530-1086	1.000000	82.2
trnG-GCC-psbZ_p4	trnG-GCC-psbZ	4	CGGATAGCGGGAATCGAAC	TTGCTTCTCCTGATGGTTGG	19	20	57.9	50.0	58.2	57.8	0.0	0.0	1.394536	24480	25381	534-1090	1.000000	80.8
trnG-GCC-psbZ_p5	trnG-GCC-psbZ	5	CGGATAGCGGGAATCGAAC	TCCTGATGGTTGGTCGAGTA	19	20	57.9	50.0	58.2	57.8	0.0	0.0	1.414896	24480	25381	527-1083	1.000000	80.7
trnS-UGA-psbC_p1	trnS-UGA-psbC	1	GGTTCGAATCCCCCTCTCT	ATTTATGGCACGCGGGAAG	19	19	57.9	52.6	58.1	58.6	0.0	15.5	2.678113	25999	26203	306-341	1.000000	70.6
trnS-UGA-psbC_p2	trnS-UGA-psbC	2	TTCGAATCCCCCTCTCTCC	ATTTATGGCACGCGGGAAG	19	19	57.9	52.6	57.8	58.6	0.0	15.5	2.821525	25999	26203	304-339	1.000000	69.4
trnS-UGA-psbC_p3	trnS-UGA-psbC	3	GGTTCGAATCCCCCTCTCTC	ATTTATGGCACGCGGGAAG	20	19	60.0	52.6	59.2	58.6	0.0	15.5	2.842050	25999	26203	306-341	1.000000	69.3
trnS-UGA-psbC_p4	trnS-UGA-psbC	4	GTTCGAATCCCCCTCTCTCC	ATTTATGGCACGCGGGAAG	20	19	60.0	52.6	59.2	58.6	0.0	15.5	2.842050	25999	26203	305-340	1.000000	69.3
trnS-UGA-psbC_p5	trnS-UGA-psbC	5	TTCGAATCCCCCTCTCTCCT	ATTTATGGCACGCGGGAAG	20	19	55.0	52.6	59.4	58.6	0.0	15.5	2.958722	25999	26203	304-339	1.000000	68.3
trnY-GUA-trnD-GUC_p1	trnY-GUA-trnD-GUC	1	TGGCAATATGTCTACGCTGG	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.8	58.5	0.0	7.1	0.716274	30214	30686	499-580	1.000000	86.3
trnY-GUA-trnD-GUC_p2	trnY-GUA-trnD-GUC	2	GGCAATATGTCTACGCTGGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.8	58.5	0.0	7.1	0.717825	30214	30686	498-579	1.000000	86.3
trnY-GUA-trnD-GUC_p3	trnY-GUA-trnD-GUC	3	GGGGACGGACTGTAAATTCG	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.828828	30214	30686	520-601	1.000000	85.4
trnY-GUA-trnD-GUC_p4	trnY-GUA-trnD-GUC	4	GGGACGGACTGTAAATTCGT	CGGTGCTCTGACCAATTGAA	20	20	50.0	50.0	57.6	58.5	0.0	7.1	0.852505	30214	30686	519-600	1.000000	85.2
trnY-GUA-trnD-GUC_p5	trnY-GUA-trnD-GUC	5	GATTCTTCCTGGGTCGATGC	CGGTGCTCTGACCAATTGAA	20	20	55.0	50.0	58.4	58.5	0.0	7.1	0.889785	30214	30686	553-634	1.000000	84.9
petN-trnC-GCA_p1	petN-trnC-GCA	1	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	20	20	45.0	50.0	58.0	58.1	0.0	0.0	0.088172	32010	32946	845-1063	1.000000	91.3
petN-trnC-GCA_p2	petN-trnC-GCA	2	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	20	20	45.0	50.0	58.0	58.4	0.0	14.7	0.361535	32010	32946	863-1081	1.000000	89.1
petN-trnC-GCA_p3	petN-trnC-GCA	3	AGAGTCCACTTCTTCCCCAT	TTTCCCCAGTTCAAATCCGG	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.432990	32010	32946	899-1117	1.000000	88.5
petN-trnC-GCA_p4	petN-trnC-GCA	4	CAGCCCAAGCAAGACTTACT	TTTCCCCAGTTCAAATCCGG	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.273256	32010	32946	908-1057	0.920000	86.6
petN-trnC-GCA_p5	petN-trnC-GCA	5	CAGCCCAAGCAAGACTTACT	GGGGGACTGCAAATCCTTTT	20	20	50.0	50.0	57.8	58.4	0.0	14.7	0.546619	32010	32946	926-1075	0.920000	84.4
trnC-GCA-rpoB_p1	trnC-GCA-rpoB	1	CCGGATTTGAACTGGGGAAA	TTCATTTCCAACCCCAAGCA	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.176228	33018	34216	1189-1357	1.000000	90.6
trnC-GCA-rpoB_p2	trnC-GCA-rpoB	2	CCGGATTTGAACTGGGGAAA	TCATTTCCAACCCCAAGCAT	20	20	50.0	45.0	58.1	57.7	0.0	0.0	0.406127	33018	34216	1188-1356	1.000000	88.8
trnC-GCA-rpoB_p3	trnC-GCA-rpoB	3	AAAAGGATTTGCAGTCCCCC	TTCATTTCCAACCCCAAGCA	20	20	50.0	45.0	58.4	57.9	14.6	0.0	0.449591	33018	34216	1171-1339	1.000000	88.4
trnC-GCA-rpoB_p4	trnC-GCA-rpoB	4	AAAAGGATTTGCAGTCCCCC	TCATTTCCAACCCCAAGCAT	20	20	50.0	45.0	58.4	57.7	14.6	0.0	0.679490	33018	34216	1170-1338	1.000000	86.6
trnC-GCA-rpoB_p5	trnC-GCA-rpoB	5	CCGGATTTGAACTGGGGAAA	CTTCATTTCCAACCCCAAGC	20	20	50.0	50.0	58.1	57.3	0.0	0.0	0.831408	33018	34216	1190-1358	1.000000	85.3
trnG-UCC-trnS-GCU_p1	trnG-UCC-trnS-GCU	1	GGATCCATAGAACGAATCGCA	GCTTTAGTCCACTCAGCCAT	21	20	47.6	50.0	58.0	57.6	7.0	0.9	1.436578	52011	52691	489-822	1.000000	80.5
trnG-UCC-trnS-GCU_p2	trnG-UCC-trnS-GCU	2	GGATCCATAGAACGAATCGCA	AGTCCACTCAGCCATCTCTC	21	20	47.6	55.0	58.0	58.5	7.0	0.0	1.542592	52011	52691	484-817	1.000000	79.7
trnG-UCC-trnS-GCU_p3	trnG-UCC-trnS-GCU	3	ACGAATCGCACTTTTACCACT	GCTTTAGTCCACTCAGCCAT	21	20	42.9	50.0	58.2	57.6	1.2	0.9	1.647203	52011	52691	478-811	1.000000	78.8
trnG-UCC-trnS-GCU_p4	trnG-UCC-trnS-GCU	4	ACGAATCGCACTTTTACCAC	GCTTTAGTCCACTCAGCCAT	20	20	45.0	50.0	56.8	57.6	1.2	0.9	1.654228	52011	52691	478-811	1.000000	78.8
trnG-UCC-trnS-GCU_p5	trnG-UCC-trnS-GCU	5	TCCATAGAACGAATCGCACT	GCTTTAGTCCACTCAGCCAT	20	20	45.0	50.0	56.8	57.6	1.2	0.9	1.658451	52011	52691	486-819	1.000000	78.7
trnQ-UUG-accD_p1	trnQ-UUG-accD	1	GAGGTTCGAATCCTTCCGTC	TCACCAACTACGGATCCCAT	20	20	55.0	50.0	58.1	58.1	16.9	6.1	0.201972	54268	54653	1073-2109	1.000000	90.4
trnQ-UUG-accD_p2	trnQ-UUG-accD	2	GAGGTTCGAATCCTTCCGTC	TCTTCGCATTGAACCCACAA	20	20	55.0	45.0	58.1	58.0	16.9	0.0	0.101836	54268	54653	737-1195	0.920000	88.0
trnQ-UUG-accD_p3	trnQ-UUG-accD	3	GAGGTTCGAATCCTTCCGTC	CTAGTACCCGTGCTTTCACC	20	20	55.0	55.0	58.1	58.0	16.9	0.0	0.082072	54268	54653	589-903	0.840000	81.9
trnQ-UUG-accD_p4	trnQ-UUG-accD	4	AGATCAGACCTCTTTCCCCC	CTAGTACCCGTGCTTTCACC	20	20	55.0	55.0	58.1	58.0	0.0	0.0	0.119548	54268	54653	902-1226	0.840000	81.1
trnQ-UUG-accD_p5	trnQ-UUG-accD	5	AGATCAGACCTCTTTCCCCC	TCTTCGCATTGAACCCACAA	20	20	55.0	45.0	58.1	58.0	0.0	0.0	0.139313	54268	54653	1068-1328	0.840000	81.0
accD_p1	accD	1	CCTTCCGTCCCAGATCCTAT	AAGAAGCCATTGCAATTGCC	20	20	55.0	45.0	58.0	57.9	0.0	26.8	0.120442	54654	55823	2050-2517	0.920000	87.0
accD_p2	accD	2	CCTTCCGTCCCAGATCCTAT	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.0	58.0	0.0	26.9	0.043908	54654	55823	2044-2511	0.880000	85.8
accD_p3	accD	3	GAGGTTCGAATCCTTCCGTC	CCATTGCAATTGCCGGAAAT	20	20	55.0	45.0	58.1	58.0	16.9	26.9	0.103104	54654	55823	2055-2522	0.880000	85.3
accD_p4	accD	4	CCTTCCGTCCCAGATCCTAT	ACTGGATGAATTTCGCCCTC	20	20	55.0	50.0	58.0	58.0	0.0	7.9	0.061027	54654	55823	1607-1876	0.800000	80.0
accD_p5	accD	5	GAGGTTCGAATCCTTCCGTC	ACTGGATGAATTTCGCCCTC	20	20	55.0	50.0	58.1	58.0	16.9	7.9	0.120224	54654	55823	1618-1887	0.800000	79.5
trnP-UGG-psaJ_p1	trnP-UGG-psaJ	1	AAAACAAACGCGCTACCAAG	ACTGGTGCCACAGAGAGATA	20	20	45.0	50.0	57.9	57.8	5.7	11.2	0.351995	62951	63358	480-665	1.000000	89.2
trnP-UGG-psaJ_p2	trnP-UGG-psaJ	2	CAAAACAAACGCGCTACCAA	ACTGGTGCCACAGAGAGATA	20	20	45.0	50.0	58.2	57.8	5.7	11.2	0.409253	62951	63358	481-666	1.000000	88.7
trnP-UGG-psaJ_p3	trnP-UGG-psaJ	3	AAAACAAACGCGCTACCAAG	CCGCTAAAATCCCGAACCAT	20	20	45.0	50.0	57.9	58.3	5.7	0.0	0.438358	62951	63358	512-697	1.000000	88.5
trnP-UGG-psaJ_p4	trnP-UGG-psaJ	4	CAAAACAAACGCGCTACCAA	CCGCTAAAATCCCGAACCAT	20	20	45.0	50.0	58.2	58.3	5.7	0.0	0.495616	62951	63358	513-698	1.000000	88.0
trnP-UGG-psaJ_p5	trnP-UGG-psaJ	5	TTGTACCCAAAACAAACGCG	ACTGGTGCCACAGAGAGATA	20	20	45.0	50.0	57.8	57.8	3.5	11.2	0.428409	62951	63358	488-673	0.880000	83.8
clpP_p1	clpP	1	CCCTCTTAGTCCGTTCTTGC	AAAAGCACAAGCCCGAAAAC	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.076951	66247	66876	1680-2532	1.000000	91.4
clpP_p2	clpP	2	CCCTCTTAGTCCGTTCTTGC	ACGAACATACCCTGTCTCCA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.078519	66247	66876	1540-2392	1.000000	91.4
clpP_p3	clpP	3	CCCTCTTAGTCCGTTCTTGC	CAAAAAGCACAAGCCCGAAA	20	20	55.0	45.0	58.0	58.1	0.0	0.0	0.078294	66247	66876	1682-1973	0.920000	88.2
clpP_p4	clpP	4	AGAAACACACGACAGCCAAT	AAAAGCACAAGCCCGAAAAC	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.098093	66247	66876	1263-2108	0.560000	73.6
clpP_p5	clpP	5	AGAAACACACGACAGCCAAT	CAAAAAGCACAAGCCCGAAA	20	20	45.0	45.0	58.0	58.1	0.0	0.0	0.099435	66247	66876	1265-1369	0.480000	70.4
trnI-CAU-ycf2_p1	trnI-CAU-ycf2	1	ATTGAACCTACGACTTCGCC	TGGTGATATTCTTCCTCCATTGA	20	23	50.0	39.1	58.0	57.0	0.0	0.0	3.980127	81844	83930	1897-2241	0.920000	57.0
trnI-CAU-ycf2_p2	trnI-CAU-ycf2	2	GAACCTACGACTTCGCCAAT	TGGTGATATTCTTCCTCCATTGA	20	23	50.0	39.1	58.0	57.0	0.0	0.0	3.980127	81844	83930	1894-2238	0.920000	57.0
trnI-CAU-ycf2_p3	trnI-CAU-ycf2	3	GTTGGGCGCTTTAACCATTC	TGGTGATATTCTTCCTCCATTGA	20	23	50.0	39.1	58.0	57.0	7.6	0.0	3.983357	81844	83930	1869-2213	0.920000	56.9
trnI-CAU-ycf2_p4	trnI-CAU-ycf2	4	AGTTGGGCGCTTTAACCATT	TGGTGATATTCTTCCTCCATTGA	20	23	45.0	39.1	58.4	57.0	7.6	0.0	4.346293	81844	83930	1870-2214	0.920000	54.0
trnI-CAU-ycf2_p5	trnI-CAU-ycf2	5	TTGGGCGCTTTAACCATTCA	TGGTGATATTCTTCCTCCATTGA	20	23	45.0	39.1	58.4	57.0	7.6	0.0	4.352164	81844	83930	1868-2212	0.920000	54.0
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	GAGGTTAAGCGTACTCTGGC	GATGACTTCCACCACGTCAA	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.239919	92404	94517	1477-2226	1.000000	90.1
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	GAGGTTAAGCGTACTCTGGC	AACTGATGACTTCCACCACG	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.245933	92404	94517	1481-2230	1.000000	90.0
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	GAGGTTAAGCGTACTCTGGC	ATGACTTCCACCACGTCAAG	20	20	55.0	50.0	58.1	57.8	0.0	0.0	0.245933	92404	94517	1476-2225	1.000000	90.0
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	GGTTAAGCGTACTCTGGCAA	GATGACTTCCACCACGTCAA	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.252768	92404	94517	1475-2224	1.000000	90.0
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	GGTTAAGCGTACTCTGGCAA	ATGACTTCCACCACGTCAAG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.258782	92404	94517	1474-2223	1.000000	89.9
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	GGCGGACCCTAAGCTATAGA	CTTTTTCCATAACCCGGGCT	20	20	55.0	50.0	58.1	58.2	3.2	24.1	0.232061	102856	103513	1046-1486	0.880000	85.3
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	GGCGGACCCTAAGCTATAGA	AAACCCAGTTGTTGCTGCTA	20	20	55.0	45.0	58.1	57.9	3.2	0.0	0.145288	102856	103513	1081-1462	0.840000	84.2
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	GGCGGACCCTAAGCTATAGA	TAAACCCAGTTGTTGCTGCT	20	20	55.0	45.0	58.1	57.9	3.2	0.0	0.145288	102856	103513	1082-1463	0.840000	84.2
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	GGCGGACCCTAAGCTATAGA	AAGAAGGCCCTACGGAGAAT	20	20	55.0	50.0	58.1	58.1	3.2	0.0	0.198449	102856	103513	1010-1391	0.840000	84.0
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	GGCGGACCCTAAGCTATAGA	ACGGTAGAGTTAGGGCAGTT	20	20	55.0	50.0	58.1	58.1	3.2	0.0	0.144980	102856	103513	1188-1618	0.800000	82.8