Hotspot

ycf3

LSC · 43069-45082 · 2014 bp

Back to Hedyotis

Pi 0.0106

MarkerSeek score 63.00

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2014
variable_sites: 32
pi: 0.0106
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	AAAACTAAAAGATGCCGGCT	ACAATTCTTTTCTTGCGCGG	40.0	45.0	55.9	57.9	2107-2111 bp	0.666667	52.0
ycf3_p2	AAACTAAAAGATGCCGGCTT	ACAATTCTTTTCTTGCGCGG	40.0	45.0	55.9	57.9	2106-2110 bp	0.666667	52.0
ycf3_p3	AACTAAAAGATGCCGGCTTT	ACAATTCTTTTCTTGCGCGG	40.0	45.0	55.9	57.9	2105-2109 bp	0.666667	52.0
ycf3_p4	ACTAAAAGATGCCGGCTTTT	ACAATTCTTTTCTTGCGCGG	40.0	45.0	55.9	57.9	2104-2108 bp	0.666667	52.0
ycf3_p5	ACTAAAAGATGCCGGCTTTT	GGGATCATTTTCTCGCGAGA	40.0	50.0	55.9	57.8	2086-2090 bp	0.666667	50.6