Hotspot

ycf1

IRb · 109539-110507 · 969 bp

Back to Hedyotis

Pi 0.0144

MarkerSeek score 41.50

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 969
variable_sites: 21
pi: 0.0144
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CGGAACTTCTTCGGAGTAGC	TTGATCGGCGTGTGATTGAT	55.0	45.0	58.1	58.0	1476-1516 bp	1.0	84.1
ycf1_p2	GTAACCCCCGGAACTTCTTC	TTGATCGGCGTGTGATTGAT	55.0	45.0	57.9	58.0	1484-1524 bp	1.0	84.0
ycf1_p3	AGGATTCGAACCTACGACCA	TTGATCGGCGTGTGATTGAT	50.0	45.0	58.2	58.0	1622-1662 bp	1.0	83.2
ycf1_p4	GGGAACGGGTTACCTACTCT	TTGATCGGCGTGTGATTGAT	55.0	45.0	58.1	58.0	1720-1760 bp	1.0	83.1
ycf1_p5	CGAGCCCCCTTTCTTTGATT	TTGATCGGCGTGTGATTGAT	50.0	45.0	58.2	58.0	1688-1728 bp	1.0	83.0