Hotspot

rrn23

IRb · 104878-107686 · 2809 bp

Back to Hedyotis

Pi 0.00783

MarkerSeek score 61.00

Primer available yes

Label rrn23

Feature Metadata

label_name: rrn23
feature_type: rRNA
region: IRb
length_bp: 2809
variable_sites: 33
pi: 0.00783
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn23_p1	TCCATCCATTTAGCAGCACC	GCCGTGACCTTCTCTTGAAT	50.0	50.0	57.9	57.9	2980-2992 bp	1.0	79.6
rrn23_p2	CCATCCATTTAGCAGCACCT	GCCGTGACCTTCTCTTGAAT	50.0	50.0	57.9	57.9	2979-2991 bp	1.0	79.6
rrn23_p3	TCCATCCATTTAGCAGCACC	AAAACTTCTGTCGCTCCGTC	50.0	50.0	57.9	58.5	2956-2968 bp	1.0	76.4
rrn23_p4	TCCATCCATTTAGCAGCACC	TCAAAACTTCTGTCGCTCCG	50.0	50.0	57.9	58.5	2958-2970 bp	1.0	76.4
rrn23_p5	CCATCCATTTAGCAGCACCT	AAAACTTCTGTCGCTCCGTC	50.0	50.0	57.9	58.5	2955-2967 bp	1.0	76.4