Hotspot

ccsA-ndhD

SSC · 115251-115547 · 297 bp

Back to Hedyotis

Pi 0.018
MarkerSeek score 64.60
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
297
variable_sites
8
pi
0.018
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 TCCCACTCAGCAATTGTAGC AGGTATTGGTATGTACCCCGA 50.0 47.6 57.9 57.7 493-509 bp 1.0 71.0
ccsA-ndhD_p2 ACTCAGCAATTGTAGCGCTT AGGTATTGGTATGTACCCCGA 45.0 47.6 58.2 57.7 489-505 bp 1.0 70.4
ccsA-ndhD_p3 GGTTCCCACTCAGCAATTGT AGGTATTGGTATGTACCCCGA 50.0 47.6 58.4 57.7 496-512 bp 1.0 68.9
ccsA-ndhD_p4 TCCCACTCAGCAATTGTAGC TCGGTTGATAAGGTCGAAGT 50.0 45.0 57.9 56.3 456-472 bp 1.0 68.2
ccsA-ndhD_p5 ACTCAGCAATTGTAGCGCTT TCGGTTGATAAGGTCGAAGT 45.0 45.0 58.2 56.3 452-468 bp 1.0 67.7