Hotspot

petB-petD

LSC · 77094-77260 · 167 bp

Back to Hedyotis

Pi 0.0579

MarkerSeek score 79.00

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 167
variable_sites: 14
pi: 0.0579
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	CCGCTTCTTACTGCCGTATT	GTTCAAGTCACACACTCCCA	50.0	50.0	58.1	57.7	263-270 bp	1.0	81.1
petB-petD_p2	AAACAGGGTATTTCTGGCCC	GTTCAAGTCACACACTCCCA	50.0	50.0	57.8	57.7	215-222 bp	1.0	80.6
petB-petD_p3	TATTGCCGCTTCTTACTGCC	GTTCAAGTCACACACTCCCA	50.0	50.0	58.3	57.7	268-275 bp	1.0	78.8
petB-petD_p4	CCGCTTCTTACTGCCGTATT	AGTTCAAGTCACACACTCCC	50.0	50.0	58.1	57.4	264-271 bp	1.0	78.7
petB-petD_p5	CCGCTTCTTACTGCCGTATT	GAATTCCAAGGCTAATGCGG	50.0	50.0	58.1	57.2	313-320 bp	1.0	76.8