Hotspot

petA-psbJ

LSC · 63518-64592 · 1075 bp

Back to Hedyotis

Pi 0.00759

MarkerSeek score 66.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1075
variable_sites: 12
pi: 0.00759
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTCTCCAAGGCCTTTTGGTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.8	57.6	1140-1211 bp	1.0	75.9
petA-psbJ_p2	TGGTCTTCTTGGCATCTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.0	57.6	1125-1196 bp	1.0	74.2
petA-psbJ_p3	TTGGTCTTCTTGGCATCTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.0	57.6	1126-1197 bp	1.0	74.2
petA-psbJ_p4	GCCTTTTGGTCTTCTTGGCA	ATTCCGGATTGGGTTCATCC	50.0	50.0	59.0	57.6	1131-1202 bp	1.0	74.2
petA-psbJ_p5	TTGGTCTTCTTGGCATCTGTT	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.8	57.6	1126-1197 bp	1.0	72.1