Hotspot

psaI-ycf4

LSC · 59770-60241 · 472 bp

Back to Hedyotis

Pi 0.0113

MarkerSeek score 63.40

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 472
variable_sites: 8
pi: 0.0113
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	TAGGCCTAGTATTTCCGGCA	GGATAAAGGCCCAGCAGAAA	50.0	50.0	57.9	57.9	584-625 bp	1.0	83.2
psaI-ycf4_p2	AGTATTTCCGGCACTTGCAA	GGATAAAGGCCCAGCAGAAA	45.0	50.0	58.1	57.9	577-618 bp	1.0	83.1
psaI-ycf4_p3	TAGGCCTAGTATTTCCGGCA	AAAGGATAAAGGCCCAGCAG	50.0	50.0	57.9	57.9	587-628 bp	1.0	83.1
psaI-ycf4_p4	AGTATTTCCGGCACTTGCAA	AAAGGATAAAGGCCCAGCAG	45.0	50.0	58.1	57.9	580-621 bp	1.0	83.1
psaI-ycf4_p5	GCACTTGCAATGGCTTCTTT	GGATAAAGGCCCAGCAGAAA	45.0	50.0	57.8	57.9	567-608 bp	1.0	82.7