Hotspot

psaA-ycf3

LSC · 42279-43068 · 790 bp

Back to Hedyotis

Pi 0.0131

MarkerSeek score 60.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 790
variable_sites: 15
pi: 0.0131
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TTTAACTTCTGGTTCCGGCG	GAAGCGCACAATTGGTTGAA	50.0	45.0	58.5	57.9	865-986 bp	1.0	79.2
psaA-ycf3_p2	TTTAACTTCTGGTTCCGGCG	AAGCGCACAATTGGTTGAAG	50.0	45.0	58.5	57.9	864-985 bp	1.0	79.2
psaA-ycf3_p3	TTTAACTTCTGGTTCCGGCG	TTGAAGATCACGAGGCGTTT	50.0	45.0	58.5	57.8	850-971 bp	1.0	79.0
psaA-ycf3_p4	TTTAACTTCTGGTTCCGGCG	TTGGTTGAAGATCACGAGGC	50.0	50.0	58.5	58.2	854-975 bp	1.0	78.8
psaA-ycf3_p5	CTGGTTCCGGCGAACTAATA	GAAGCGCACAATTGGTTGAA	50.0	45.0	57.4	57.9	857-978 bp	1.0	78.4