Hotspot

psbC-trnS-UGA

LSC · 35737-36005 · 269 bp

Back to Hedyotis

Pi 0.0177

MarkerSeek score 66.20

Primer available yes

Label psbC-trnS-UGA

Feature Metadata

label_name: psbC-trnS-UGA
feature_type: igs
region: LSC
length_bp: 269
variable_sites: 7
pi: 0.0177
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS-UGA_p1	GCAGCAGGGTTTGAAAAAGG	GGTGGCTGAGTGGTTGATAG	50.0	55.0	58.1	58.0	422-444 bp	1.0	81.3
psbC-trnS-UGA_p2	GCAGCAGGGTTTGAAAAAGG	ATAGCCCCGGTCTTGAAAAC	50.0	50.0	58.1	57.9	406-428 bp	1.0	80.7
psbC-trnS-UGA_p3	TCGTAGGTCATTTGTGGCAC	GGTGGCTGAGTGGTTGATAG	50.0	55.0	58.2	58.0	463-485 bp	1.0	80.6
psbC-trnS-UGA_p4	GCAGCAGGGTTTGAAAAAGG	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.1	58.2	357-379 bp	1.0	80.3
psbC-trnS-UGA_p5	TCGTAGGTCATTTGTGGCAC	ATAGCCCCGGTCTTGAAAAC	50.0	50.0	58.2	57.9	447-469 bp	1.0	79.9