Hotspot

atpH-atpI

LSC · 14227-14931 · 705 bp

Back to Hedyotis

Pi 0.0109

MarkerSeek score 70.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 705
variable_sites: 11
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAAACCAGCAGCAATAACGG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.0	58.5	806-1002 bp	1.0	79.4
atpH-atpI_p2	CCAAACCAGCAGCAATAACG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	58.0	58.5	807-1003 bp	1.0	79.4
atpH-atpI_p3	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	793-989 bp	1.0	79.3
atpH-atpI_p4	AAACCAGCAGCAATAACGGA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	805-1001 bp	1.0	78.8
atpH-atpI_p5	AACCAGCAGCAATAACGGAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	804-1000 bp	1.0	78.8