Hotspot

rps16-trnQ-UUG

LSC · 6065-7561 · 1497 bp

Back to Hedyotis

Pi 0.0113
MarkerSeek score 59.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1497
variable_sites
24
pi
0.0113
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1382-1570 bp 1.0 83.6
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA AAAAATCGAAATGGGGCGTG 45.0 45.0 58.1 57.6 1441-1629 bp 1.0 81.2
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1386-1574 bp 1.0 78.8
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1412-1600 bp 1.0 78.7
rps16-trnQ-UUG_p5 CGTTGCTTTCTACCACATCG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.2 58.1 1374-1562 bp 1.0 78.4