Hotspot

rps19

LSC · 87010-87288 · 279 bp

Back to Hedyosmum

Pi 0.0155

MarkerSeek score 39.70

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 279
variable_sites: 10
pi: 0.0155
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	TTTTCACGAAAGCACCCTCA	GTTATCCTGCGCTTGGAAGA	45.0	50.0	58.0	58.0	913-970 bp	1.0	89.9
rps19_p2	TTTTCACGAAAGCACCCTCA	GGGTAGAGCCGGATCTAAGT	45.0	55.0	58.0	58.0	1050-1107 bp	1.0	89.3
rps19_p3	TTTTCACGAAAGCACCCTCA	TGGGTAGAGCCGGATCTAAG	45.0	55.0	58.0	58.0	1051-1108 bp	1.0	89.3
rps19_p4	TTTTCACGAAAGCACCCTCA	GAGCCGGATCTAAGTGTTGG	45.0	55.0	58.0	58.1	1045-1102 bp	1.0	88.8
rps19_p5	TTTTCACGAAAGCACCCTCA	GAGGGTAAATCGGCCACATT	45.0	50.0	58.0	57.9	1163-1220 bp	1.0	88.4