Hotspot

rpl2

IRa · 156601-158086 · 1486 bp

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Pi 0.00187

MarkerSeek score 35.10

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRa
length_bp: 1486
variable_sites: 9
pi: 0.00187
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	GAATGGTTAAAGCGCCCAAC	CGTTCGCCCATCATTCCATA	50.0	50.0	58.0	58.1	2038-2043 bp	1.0	79.7
rpl2_p2	GAATGGTTAAAGCGCCCAAC	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.0	58.1	2103-2108 bp	0.625	64.5
rpl2_p3	GAATGGTTAAAGCGCCCAAC	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.0	57.9	2089-2094 bp	0.625	64.5
rpl2_p4	GAATGGTTAAAGCGCCCAAC	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.0	57.9	2088-2093 bp	0.625	64.5
rpl2_p5	GAATGGTTAAAGCGCCCAAC	GTGGATCAAGGCAGTGGATT	50.0	50.0	58.0	57.9	2090-2095 bp	0.625	64.5