Hotspot

ndhD

SSC · 124792-126294 · 1503 bp

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Pi 0.0115

MarkerSeek score 48.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 43
pi: 0.0115
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TATGGCATGAGACAACTCGC	AGGTGCAAATTCGGCAATTG	50.0	45.0	58.1	57.9	2045-2074 bp	1.0	82.5
ndhD_p2	TATGGCATGAGACAACTCGC	CAAGGTGCAAATTCGGCAAT	50.0	45.0	58.1	57.9	2047-2076 bp	1.0	82.5
ndhD_p3	CTGCGTAGGTTGTAAGCGAT	AGGTGCAAATTCGGCAATTG	50.0	45.0	58.1	57.9	2110-2139 bp	1.0	82.3
ndhD_p4	TATGGCATGAGACAACTCGC	AGGAACATCGTCCCGTAGAT	50.0	50.0	58.1	57.9	1899-1928 bp	0.875	77.5
ndhD_p5	CTGCGTAGGTTGTAAGCGAT	AGGAACATCGTCCCGTAGAT	50.0	50.0	58.1	57.9	1964-1993 bp	0.875	77.2