Hotspot

rpl32-ndhF

SSC · 128599-129571 · 973 bp

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Pi 0.0313

MarkerSeek score 71.20

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 973
variable_sites: 73
pi: 0.0313
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACCAGTCAAGCCAGTCCTAT	ACGAAAGGTATGACCCATGC	50.0	50.0	58.0	58.0	968-1263 bp	1.0	89.2
rpl32-ndhF_p2	ACCAGTCAAGCCAGTCCTAT	GGAACAAGCAGAAGTCCCAA	50.0	50.0	58.0	58.0	1016-1311 bp	1.0	89.1
rpl32-ndhF_p3	ACCAGTCAAGCCAGTCCTAT	GAAAGGTATGACCCATGCGT	50.0	50.0	58.0	58.0	966-1246 bp	0.625	71.6
rpl32-ndhF_p4	TCAAAATTGCATCACGGCAC	GGAACAAGCAGAAGTCCCAA	45.0	50.0	57.9	58.0	1749-1755 bp	0.25	49.7
rpl32-ndhF_p5	TCAAAATTGCATCACGGCAC	GAAAGGTATGACCCATGCGT	45.0	50.0	57.9	58.0	1699-1705 bp	0.25	49.5