Hotspot

ndhD-ccsA

SSC · 126295-126553 · 259 bp

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Pi 0.0516

MarkerSeek score 68.30

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 259
variable_sites: 34
pi: 0.0516
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	GGACCGCGAGAACTATTTGT	AGGTGCAAATTCGGCAATTG	50.0	45.0	58.0	57.9	514-539 bp	1.0	87.1
ndhD-ccsA_p2	GGACCGCGAGAACTATTTGT	CAAGGTGCAAATTCGGCAAT	50.0	45.0	58.0	57.9	516-541 bp	1.0	87.1
ndhD-ccsA_p3	TGGACCGCGAGAACTATTTG	AGGTGCAAATTCGGCAATTG	50.0	45.0	58.0	57.9	515-533 bp	0.875	81.5
ndhD-ccsA_p4	TGGACCGCGAGAACTATTTG	CAAGGTGCAAATTCGGCAAT	50.0	45.0	58.0	57.9	517-535 bp	0.875	81.5
ndhD-ccsA_p5	TCGCTATCAGTTGACAAGGC	AGGTGCAAATTCGGCAATTG	50.0	45.0	58.0	57.9	436-461 bp	0.875	80.9