Hotspot

rps19-trnH-GUG

IRb · 87289-87462 · 174 bp

Back to Hedyosmum

Pi 0.106

MarkerSeek score 71.70

Primer available yes

Label rps19-trnH-GUG

Feature Metadata

label_name: rps19-trnH-GUG
feature_type: igs
region: IRb
length_bp: 174
variable_sites: 41
pi: 0.106
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-trnH-GUG_p1	TGTCCCCGGAAAGTAAGAGT	GGGTAGAGCCGGATCTAAGT	50.0	55.0	58.0	58.0	673-728 bp	1.0	91.8
rps19-trnH-GUG_p2	GATGTCCGGGACCAAGTTAC	GGGTAGAGCCGGATCTAAGT	55.0	55.0	58.0	58.0	538-593 bp	1.0	91.8
rps19-trnH-GUG_p3	TGTCCCCGGAAAGTAAGAGT	TGGGTAGAGCCGGATCTAAG	50.0	55.0	58.0	58.0	674-729 bp	1.0	91.8
rps19-trnH-GUG_p4	GATGTCCGGGACCAAGTTAC	TGGGTAGAGCCGGATCTAAG	55.0	55.0	58.0	58.0	539-594 bp	1.0	91.8
rps19-trnH-GUG_p5	TGTATGGCCGATCATTGTGG	GGGTAGAGCCGGATCTAAGT	50.0	55.0	58.0	58.0	569-624 bp	1.0	91.7