Hotspot

rpl14-rpl16

LSC · 84174-84319 · 146 bp

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Pi 0.0447
MarkerSeek score 74.10
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
146
variable_sites
17
pi
0.0447
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.94
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 GTCTCAATTCCCGAGCGATT ATACGCTCTTCAGGCACTTG 50.0 50.0 58.1 58.0 772-786 bp 1.0 84.4
rpl14-rpl16_p2 GTCTCAATTCCCGAGCGATT CGTTACAGTAAGACCCACCG 50.0 55.0 58.1 58.0 658-660 bp 0.25 49.7
rpl14-rpl16_p3 GTCTCAATTCCCGAGCGATT ACCCGTTACAGTAAGACCCA 50.0 50.0 58.1 58.0 661-663 bp 0.25 49.7
rpl14-rpl16_p4 TCTCAATTCCCGAGCGATTG CGTTACAGTAAGACCCACCG 50.0 55.0 58.1 58.0 657-659 bp 0.25 49.7
rpl14-rpl16_p5 TCTCAATTCCCGAGCGATTG ACCCGTTACAGTAAGACCCA 50.0 50.0 58.1 58.0 660-662 bp 0.25 49.6