Hotspot

psbB-psbT

LSC · 77035-77229 · 195 bp

Back to Hedyosmum

Pi 0.0361

MarkerSeek score 74.30

Primer available yes

Label psbB-psbT

Feature Metadata

label_name: psbB-psbT
feature_type: igs
region: LSC
length_bp: 195
variable_sites: 17
pi: 0.0361
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-psbT_p1	GGATGCTCAAGTGGAATTTGG	CCTAGGCGGTTCTCGAAAAA	47.6	50.0	57.5	57.9	347-374 bp	1.0	71.9
psbB-psbT_p2	GGCGCTAGAACCTTATTCAGA	CCTAGGCGGTTCTCGAAAAA	47.6	50.0	57.5	57.9	397-424 bp	1.0	71.8
psbB-psbT_p3	AGATTTGGATGCTCAAGTGGA	CCTAGGCGGTTCTCGAAAAA	42.9	50.0	57.3	57.9	353-380 bp	1.0	70.1
psbB-psbT_p4	CAGATTTGGATGCTCAAGTGG	CCTAGGCGGTTCTCGAAAAA	47.6	50.0	57.2	57.9	354-381 bp	1.0	69.7
psbB-psbT_p5	GGCGCTAGAACCTTATTCAG	CCTAGGCGGTTCTCGAAAAA	50.0	50.0	56.0	57.9	397-424 bp	1.0	67.6