Hotspot

rps18-rpl20

LSC · 71414-71682 · 269 bp

Back to Hedyosmum

Pi 0.0334

MarkerSeek score 66.80

Primer available yes

Label rps18-rpl20

Feature Metadata

label_name: rps18-rpl20
feature_type: igs
region: LSC
length_bp: 269
variable_sites: 26
pi: 0.0334
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps18-rpl20_p1	CAATAAGGAATCACCGGGCA	CGTTTGTGGATCACTCGGAT	50.0	50.0	57.9	58.0	1020-1031 bp	1.0	83.8
rps18-rpl20_p2	CAATAAGGAATCACCGGGCA	TCGTCGTTTGTGGATCACTC	50.0	50.0	57.9	57.9	1024-1035 bp	1.0	83.4
rps18-rpl20_p3	CAATAAGGAATCACCGGGCA	TTTCGAGGGGCTCATTCAAG	50.0	50.0	57.9	57.9	1125-1136 bp	1.0	82.8
rps18-rpl20_p4	CAATAAGGAATCACCGGGCA	GACGTCGAACAAAAATGCGT	50.0	45.0	57.9	58.0	1160-1171 bp	0.625	66.2
rps18-rpl20_p5	CAATAAGGAATCACCGGGCA	GGGTAGAGCCAAAGACTGTG	50.0	55.0	57.9	57.9	1563-1565 bp	0.25	48.9