Hotspot

psbK-psbI

LSC · 8079-8481 · 403 bp

Back to Hedyosmum

Pi 0.0245

MarkerSeek score 63.80

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 403
variable_sites: 27
pi: 0.0245
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	GGATTACGTCCCGGATCATT	50.0	50.0	57.9	57.2	517-538 bp	1.0	80.5
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TCCTCACGTCCAGGATTACG	50.0	55.0	57.9	58.9	529-550 bp	1.0	79.8
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	GGATTACGTCCCGGATCATT	45.0	50.0	58.3	57.2	514-535 bp	1.0	78.5
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	GGATTACGTCCCGGATCATT	45.0	50.0	58.3	57.2	515-536 bp	1.0	78.5
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	GGATTACGTCCCGGATCATT	45.0	50.0	58.3	57.2	516-537 bp	1.0	78.5