Hotspot

rps16-trnQ-UUG

LSC · 5569-7504 · 1936 bp

Back to Hedyosmum

Pi 0.0243
MarkerSeek score 67.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1936
variable_sites
128
pi
0.0243
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 2005-2057 bp 1.0 84.0
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 2009-2061 bp 1.0 82.9
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TACTCGGAGGTTCGAATCCT 45.0 50.0 58.1 57.6 2011-2063 bp 1.0 81.1
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 2019-2068 bp 0.75 72.8
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 2023-2072 bp 0.75 71.7