Hotspot

ycf1

SSC · 113938-117933 · 3996 bp

Back to Hedychium

Pi 0.0039

MarkerSeek score 49.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3996
variable_sites: 134
pi: 0.0039
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCACTTACTGAAGAATCACCA	ATTGGGATTGGATGTGTACT	40.9	40.0	56.8	54.0	187 bp	0.05	2.0
ycf1_p2	TCCACTTACTGAAGAATCACCA	AATTGGGATTGGATGTGTACT	40.9	38.1	56.8	54.9	188 bp	0.05	2.0
ycf1_p3	TCCACTTACTGAAGAATCACCA	AAATTGGGATTGGATGTGTACT	40.9	36.4	56.8	55.7	189 bp	0.05	2.0
ycf1_p4	TTCCACTTACTGAAGAATCACCA	ATTGGGATTGGATGTGTACT	39.1	40.0	57.4	54.0	188 bp	0.05	2.0
ycf1_p5	TTCCACTTACTGAAGAATCACCA	AATTGGGATTGGATGTGTACT	39.1	38.1	57.4	54.9	189 bp	0.05	2.0