Hotspot

psbA

LSC · 218-1279 · 1062 bp

Back to Hedychium

Pi 0.000763

MarkerSeek score 35.90

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 3
pi: 0.000763
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACCCCATATCTCGCTTAAGC	ACATATGCTTGGGAGTCCTT	50.0	45.0	57.2	56.2	1203-1204 bp	1.0	59.1
psbA_p2	GCTTAAGCGAGATATGGGGT	ACATATGCTTGGGAGTCCTT	50.0	45.0	57.2	56.2	1191-1192 bp	1.0	59.1
psbA_p3	ACCCCATATCTCGCTTAAGC	TACATATGCTTGGGAGTCCT	50.0	45.0	57.2	55.3	1204-1205 bp	1.0	51.9
psbA_p4	GCTTAAGCGAGATATGGGGT	TACATATGCTTGGGAGTCCT	50.0	45.0	57.2	55.3	1192-1193 bp	1.0	51.9
psbA_p5	GCTTAAGCGAGATATGGGGT	ACATATGCTTGGGAGTCCTTA	50.0	42.9	57.2	56.1	1191-1192 bp	1.0	50.5