Hotspot

ndhD

SSC · 123413-124918 · 1506 bp

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Pi 0.00106

MarkerSeek score 34.50

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1506
variable_sites: 7
pi: 0.00106
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	GGCGTTTAAAAGCGGAAAAT	TACGGGCTTTTGTGGTCAAA	40.0	45.0	55.8	57.9	1604-1605 bp	1.0	62.7
ndhD_p2	GGCGTTTAAAAGCGGAAAAT	AAGTACGGGCTTTTGTGGTC	40.0	50.0	55.8	58.4	1607-1608 bp	1.0	59.9
ndhD_p3	GGCGTTTAAAAGCGGAAAAT	GTACGGGCTTTTGTGGTCAA	40.0	50.0	55.8	58.7	1605-1606 bp	1.0	57.6
ndhD_p4	GGCGTTTAAAAGCGGAAAAT	ACGGGCTTTTGTGGTCAAAA	40.0	45.0	55.8	58.8	1603-1604 bp	1.0	56.6
ndhD_p5	GGCGTTTAAAAGCGGAAAAT	TAAGTACGGGCTTTTGTGGT	40.0	45.0	55.8	57.1	1608-1609 bp	1.0	55.6