Hotspot

rpl2-trnH-GUG

IRa · 162698-162744 · 47 bp

Back to Hedychium

Pi 0.0183

MarkerSeek score 57.80

Primer available yes

Label rpl2-trnH-GUG

Feature Metadata

label_name: rpl2-trnH-GUG
feature_type: igs
region: IRa
length_bp: 47
variable_sites: 5
pi: 0.0183
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-trnH-GUG_p1	GTTATCCTGCGCTTGGAAGA	TGGATCAAGGCAGTGGATTG	50.0	50.0	58.0	57.9	182-184 bp	1.0	81.4
rpl2-trnH-GUG_p2	GTTATCCTGCGCTTGGAAGA	GGATCAAGGCAGTGGATTGT	50.0	50.0	58.0	57.9	181-183 bp	1.0	81.4
rpl2-trnH-GUG_p3	GTTATCCTGCGCTTGGAAGA	GTGGATCAAGGCAGTGGATT	50.0	50.0	58.0	57.9	183-185 bp	1.0	81.3
rpl2-trnH-GUG_p4	GTTATCCTGCGCTTGGAAGA	CGGATGTAGCCAAGTGGATC	50.0	55.0	58.0	58.1	196-198 bp	1.0	81.2
rpl2-trnH-GUG_p5	GTTATCCTGCGCTTGGAAGA	TAGCTAGAAAAGGGGCGGAT	50.0	50.0	58.0	58.2	211-213 bp	1.0	80.5