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psaC-ndhE

SSC · 125289-125810 · 522 bp

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Pi 0.00648

MarkerSeek score 60.40

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 522
variable_sites: 31
pi: 0.00648
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CGTCCCACGGTATCATTTCC	AACGCGTATCAACCAATCGA	55.0	45.0	58.4	58.0	656-910 bp	1.0	83.7
psaC-ndhE_p2	CGTCCCACGGTATCATTTCC	ACGCGTATCAACCAATCGAA	55.0	45.0	58.4	58.0	655-909 bp	1.0	83.7
psaC-ndhE_p3	CGTCCCACGGTATCATTTCC	CAACGCGTATCAACCAATCG	55.0	50.0	58.4	57.9	657-911 bp	1.0	83.1
psaC-ndhE_p4	GGCAAGCTCGTACACATTGA	AACGCGTATCAACCAATCGA	50.0	45.0	58.6	58.0	623-877 bp	1.0	82.7
psaC-ndhE_p5	GGCAAGCTCGTACACATTGA	ACGCGTATCAACCAATCGAA	50.0	45.0	58.6	58.0	622-876 bp	1.0	82.7