Hotspot

ccsA-ndhD

SSC · 123141-123412 · 272 bp

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Pi 0.0123

MarkerSeek score 54.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 272
variable_sites: 10
pi: 0.0123
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGTGGCTTCTATGGGATTCC	TCCCGATTTCATTCTCTCGT	50.0	45.0	57.0	56.4	452-507 bp	1.0	62.5
ccsA-ndhD_p2	TGTGGCTTCTATGGGATTCC	TCCCGATTTCATTCTCTCGTT	50.0	42.9	57.0	57.1	452-507 bp	1.0	60.5
ccsA-ndhD_p3	TGTGGCTTCTATGGGATTCC	GGGACTGGTATTTATCCCGA	50.0	50.0	57.0	56.1	466-521 bp	1.0	60.3
ccsA-ndhD_p4	TTGTGGCTTCTATGGGATTCC	TCCCGATTTCATTCTCTCGT	47.6	45.0	57.7	56.4	453-508 bp	1.0	60.3
ccsA-ndhD_p5	GTGGCTTCTATGGGATTCCT	TCCCGATTTCATTCTCTCGT	50.0	45.0	56.7	56.4	451-506 bp	1.0	60.1