Hotspot

ndhF-rpl32

SSC · 120173-120950 · 778 bp

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Pi 0.00492
MarkerSeek score 47.00
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
778
variable_sites
15
pi
0.00492
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.84
markerseek_score
47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CGGAATAAGTAGAAGCCCGA AGAAAAAGCCTTTACTGCAGC 50.0 42.9 56.8 57.3 861-978 bp 1.0 62.8
ndhF-rpl32_p2 CGGAATAAGTAGAAGCCCGA GAAAAAGCCTTTACTGCAGCT 50.0 42.9 56.8 57.3 860-977 bp 1.0 62.8
ndhF-rpl32_p3 TCGGAATAAGTAGAAGCCCG AGAAAAAGCCTTTACTGCAGC 50.0 42.9 56.8 57.3 862-979 bp 1.0 62.8
ndhF-rpl32_p4 TCGGAATAAGTAGAAGCCCG GAAAAAGCCTTTACTGCAGCT 50.0 42.9 56.8 57.3 861-978 bp 1.0 62.8
ndhF-rpl32_p5 GAAGCCCGAATCCTATTGACA GAAAAAGCCTTTACTGCAGCT 47.6 42.9 57.8 57.3 849-966 bp 1.0 62.6