Hotspot

accD-psaI

LSC · 62239-62759 · 521 bp

Back to Hedychium

Pi 0.00437

MarkerSeek score 58.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 21
pi: 0.00437
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TCGACCCAATAGTACCACGT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.2	58.0	677-742 bp	1.0	84.9
accD-psaI_p2	TCGACCCAATAGTACCACGT	AGAAACCATTGCAATTGCCG	50.0	45.0	58.2	57.9	682-747 bp	1.0	84.4
accD-psaI_p3	TTCGACCCAATAGTACCACG	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.1	58.0	678-743 bp	1.0	78.6
accD-psaI_p4	TTCGACCCAATAGTACCACG	AGAAACCATTGCAATTGCCG	50.0	45.0	57.1	57.9	683-748 bp	1.0	78.1
accD-psaI_p5	TCGACCCAATAGTACCACGTA	CCATTGCAATTGCCGGAAAT	47.6	45.0	58.0	58.0	677-742 bp	1.0	78.1