Hotspot

ndhC-trnV-UAC

LSC · 52895-54604 · 1710 bp

Back to Hedychium

Pi 0.00643

MarkerSeek score 55.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1710
variable_sites: 34
pi: 0.00643
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 55.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCTAACCGGGGCTAAAACTC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	1232-1848 bp	1.0	83.9
ndhC-trnV-UAC_p2	CTAACCGGGGCTAAAACTCC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.0	57.8	1231-1847 bp	1.0	83.9
ndhC-trnV-UAC_p3	GGGGCTAAAACTCCGGAAAT	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	1225-1841 bp	1.0	83.1
ndhC-trnV-UAC_p4	CCTAACCGGGGCTAAAACTC	AGCCCAATGCAACAAAATGC	55.0	45.0	58.0	58.5	1314-1930 bp	1.0	81.6
ndhC-trnV-UAC_p5	CTAACCGGGGCTAAAACTCC	AGCCCAATGCAACAAAATGC	55.0	45.0	58.0	58.5	1313-1929 bp	1.0	81.6