Pi
0.00509
MarkerSeek score
48.60
Primer available
yes
Label
rps4-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| rps4-trnL-UAA_p1 | TCACTTCCGGATCTAGGTCT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 56.9 | 58.0 | 1179-1695 bp | 1.0 | 73.8 |
| rps4-trnL-UAA_p2 | TCACTTCCGGATCTAGGTCT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 56.9 | 58.1 | 1187-1703 bp | 1.0 | 73.2 |
| rps4-trnL-UAA_p3 | ACCAGAACGCAATTGGTTTT | ACCAATTTCGCCATATCCCC | 40.0 | 50.0 | 56.7 | 58.0 | 1204-1720 bp | 1.0 | 72.6 |
| rps4-trnL-UAA_p4 | ACCAGAACGCAATTGGTTTT | TAGCGTCTACCAATTTCGCC | 40.0 | 50.0 | 56.7 | 58.1 | 1212-1728 bp | 1.0 | 72.1 |
| rps4-trnL-UAA_p5 | TCACTTCCGGATCTAGGTCT | ATCCAATCAAGTCCGTAGCG | 50.0 | 50.0 | 56.9 | 57.8 | 1202-1718 bp | 1.0 | 72.0 |