Hotspot

trnT-GGU-psbD

LSC · 34156-35435 · 1280 bp

Back to Hedychium

Pi 0.00906

MarkerSeek score 60.40

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1280
variable_sites: 46
pi: 0.00906
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	CCCACAAAAACGAAACGGTC	55.0	50.0	58.1	58.2	1426-1483 bp	1.0	83.2
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	CCCACAAAAACGAAACGGTC	50.0	50.0	58.1	58.2	1429-1486 bp	1.0	83.0
trnT-GGU-psbD_p3	GTGGTAGAGTAACGCCATGG	AACCCACAAAAACGAAACGG	55.0	45.0	58.1	57.7	1428-1485 bp	1.0	82.3
trnT-GGU-psbD_p4	TCAGTGGTAGAGTAACGCCA	AACCCACAAAAACGAAACGG	50.0	45.0	58.1	57.7	1431-1488 bp	1.0	82.1
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	GGTCTCTGCGTAACCAATCA	55.0	50.0	58.1	57.6	1410-1467 bp	1.0	81.6