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atpH-atpI

LSC · 14323-15426 · 1104 bp

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Pi 0.00264

MarkerSeek score 57.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1104
variable_sites: 23
pi: 0.00264
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	ATTTTTGCAACCTTAGCCGC	45.0	45.0	58.0	57.9	1157-1678 bp	1.0	84.2
atpH-atpI_p2	TACCTTCTACAGCTTGGCCT	ATTTTTGCAACCTTAGCCGC	50.0	45.0	58.0	57.9	1240-1761 bp	1.0	84.2
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	ATTTTTGCAACCTTAGCCGC	50.0	45.0	57.9	57.9	1213-1734 bp	1.0	83.7
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	ATTTTTGCAACCTTAGCCGC	45.0	45.0	58.2	57.9	1158-1679 bp	1.0	83.1
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	ATTTTTGCAACCTTAGCCGC	55.0	45.0	58.2	57.9	1217-1738 bp	1.0	83.0