Hotspot

ndhD

SSC · 120753-122255 · 1503 bp

Back to Hedlundia

Pi 0.00222

MarkerSeek score 50.90

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 7
pi: 0.00222
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	ATCATGGGATTTCAGTTTCA	CTTTACCGACAAAAACCCGC	35.0	50.0	52.4	58.0	1626 bp	1.0	40.3
ndhD_p2	TCATGGGATTTCAGTTTCAT	CTTTACCGACAAAAACCCGC	35.0	50.0	52.4	58.0	1625 bp	1.0	40.3
ndhD_p3	AATCATGGGATTTCAGTTTCA	CTTTACCGACAAAAACCCGC	33.3	50.0	53.3	58.0	1627 bp	1.0	40.3
ndhD_p4	TCATGGGATTTCAGTTTCATT	CTTTACCGACAAAAACCCGC	33.3	50.0	53.3	58.0	1625 bp	1.0	40.3
ndhD_p5	TCATGGGATTTCAGTTTCATTT	CTTTACCGACAAAAACCCGC	31.8	50.0	54.2	58.0	1625 bp	1.0	40.3