Hotspot

psaJ-rpl33

LSC · 70759-71222 · 464 bp

Back to Hedlundia

Pi 0.00216

MarkerSeek score 39.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 464
variable_sites: 3
pi: 0.00216
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TCCGGATGCGTTGATATTCC	CACGATTTCGAACACAACTGG	50.0	47.6	57.8	58.1	555-577 bp	1.0	72.3
psaJ-rpl33_p2	TCCGGATGCGTTGATATTCC	TGGAAATGCCCATTGATTCCT	50.0	42.9	57.8	57.9	582-604 bp	1.0	72.0
psaJ-rpl33_p3	TCCGGATGCGTTGATATTCC	CGGACATCTTTACCCTTAGCC	50.0	52.4	57.8	58.2	511-533 bp	1.0	72.0
psaJ-rpl33_p4	TCCGGATGCGTTGATATTCC	ACGATTTCGAACACAACTGG	50.0	45.0	57.8	56.7	554-576 bp	1.0	70.5
psaJ-rpl33_p5	TCCGGATGCGTTGATATTCC	CGATTTCGAACACAACTGGT	50.0	45.0	57.8	56.7	553-575 bp	1.0	70.5