Hotspot

ycf3-trns-GGA

LSC · 47302-47914 · 613 bp

Back to Hedlundia

Pi 0.00109

MarkerSeek score 50.70

Primer available yes

Label ycf3-trns-GGA

Feature Metadata

label_name: ycf3-trns-GGA
feature_type: igs
region: LSC
length_bp: 613
variable_sites: 2
pi: 0.00109
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trns-GGA_p1	TGCCTCTTTTTCTCCCGAAG	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	805-806 bp	1.0	77.7
ycf3-trns-GGA_p2	GCCTCTTTTTCTCCCGAAGT	ACGGAAAGAGAGGGATTCGA	50.0	50.0	57.8	58.2	804-805 bp	1.0	77.7
ycf3-trns-GGA_p3	TGCCTCTTTTTCTCCCGAAG	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	755-756 bp	1.0	77.4
ycf3-trns-GGA_p4	GCCTCTTTTTCTCCCGAAGT	TTCCAATGCTACGCCTTGAA	50.0	45.0	57.8	57.8	754-755 bp	1.0	77.4
ycf3-trns-GGA_p5	TGCCTCTTTTTCTCCCGAAG	AGTTCCAATGCTACGCCTTG	50.0	50.0	57.8	58.5	757-758 bp	1.0	74.6