Hotspot

rpoB-trnC-GCA

LSC · 27874-29091 · 1218 bp

Back to Hedlundia

Pi 0.00317

MarkerSeek score 61.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1218
variable_sites: 9
pi: 0.00317
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	TAAGCCCTGCTCAATGAACC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1367-1375 bp	1.0	79.7
rpoB-trnC-GCA_p2	TAAGCCCTGCTCAATGAACC	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.9	58.4	1349-1357 bp	1.0	77.5
rpoB-trnC-GCA_p3	TATTGCCTCATTTCCACCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1301-1309 bp	1.0	77.1
rpoB-trnC-GCA_p4	CTCTGTTAAGCCCTGCTCAA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.5	58.1	1373-1381 bp	1.0	76.8
rpoB-trnC-GCA_p5	GTTCCTCTGTTAAGCCCTGC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.5	58.1	1377-1385 bp	1.0	76.3