Hotspot

trnK-UUU-rps16

LSC · 4353-5294 · 942 bp

Back to Hedlundia

Pi 0.00354
MarkerSeek score 55.10
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
942
variable_sites
8
pi
0.00354
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT CGGAACTTCGCCCTAATCAA 50.0 50.0 58.2 58.0 1014-1029 bp 1.0 81.1
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT CGGAACTTCGCCCTAATCAA 50.0 50.0 58.2 58.0 1013-1028 bp 1.0 81.1
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA CGGAACTTCGCCCTAATCAA 50.0 50.0 58.3 58.0 1023-1038 bp 1.0 80.1
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1069-1084 bp 1.0 79.6
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT GGTGCTCAACCTACAGGAAC 50.0 55.0 58.2 58.2 1068-1083 bp 1.0 79.6