Hotspot

ndhA

SSC · 122404-124564 · 2161 bp

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Pi 0.001
MarkerSeek score 48.70
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2161
variable_sites
4
pi
0.001
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TGTTTGAGACAAGTCGTGAAGA CACCGGGTTTTATCAATTTGCA 40.9 40.9 57.9 58.1 2340-2341 bp 1.0 46.6
ndhA_p2 TGTTTGAGACAAGTCGTGAAGA ACCGGGTTTTATCAATTTGCA 40.9 38.1 57.9 56.7 2339-2340 bp 1.0 44.7
ndhA_p3 TGTTTGAGACAAGTCGTGAAG CACCGGGTTTTATCAATTTGCA 42.9 40.9 56.4 58.1 2340-2341 bp 1.0 43.1
ndhA_p4 TGTTTGAGACAAGTCGTGAAGA ACCGGGTTTTATCAATTTGCAA 40.9 36.4 57.9 57.4 2339-2340 bp 1.0 42.4
ndhA_p5 TGTTTGAGACAAGTCGTGAAGA CACCGGGTTTTATCAATTTGC 40.9 42.9 57.9 56.4 2340-2341 bp 1.0 42.2