Hotspot

ndhB-trnL-CAA

IRa · 146693-147270 · 578 bp

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Pi 0
MarkerSeek score 35.20
Primer available yes
Label ndhB-trnL-CAA

Feature Metadata

label_name
ndhB-trnL-CAA
feature_type
igs
region
IRa
length_bp
578
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhB-trnL-CAA_p1 TGCAATTGCTCAGGATACCC GTCTCGCGTGTCTACCATTT 50.0 50.0 57.9 58.0 638 bp 1.0 79.5
ndhB-trnL-CAA_p2 TGCAATTGCTCAGGATACCC CTCGCGTGTCTACCATTTCA 50.0 50.0 57.9 58.0 636 bp 1.0 79.5
ndhB-trnL-CAA_p3 TGCAATTGCTCAGGATACCC TACCATTTCACCACCAAGGC 50.0 50.0 57.9 58.1 626 bp 1.0 78.9
ndhB-trnL-CAA_p4 TGCAATTGCTCAGGATACCC GCGTGTCTACCATTTCACCA 50.0 50.0 57.9 58.2 633 bp 1.0 78.0
ndhB-trnL-CAA_p5 TGCAATTGCTCAGGATACCC TCTCGCGTGTCTACCATTTC 50.0 50.0 57.9 57.7 637 bp 1.0 77.4