Hotspot

rpl32-trnL-UAG

SSC · 116088-116900 · 813 bp

Back to Hedera

Pi 0.00144
MarkerSeek score 51.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
813
variable_sites
2
pi
0.00144
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGGATATTGGGCAGCCTTA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 934-940 bp 1.0 79.3
rpl32-trnL-UAG_p2 AGGGATATTGGGCAGCCTTA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 935-941 bp 1.0 79.3
rpl32-trnL-UAG_p3 AGGGATATTGGGCAGCCTTA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.2 57.9 944-950 bp 1.0 78.3
rpl32-trnL-UAG_p4 GCAGCCTTAAAAGCGTTGTC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.3 58.0 923-929 bp 1.0 78.1
rpl32-trnL-UAG_p5 GCAGCCTTAAAAGCGTTGTC CAGCGTGTCTACCGATTTCA 50.0 50.0 58.3 58.0 924-930 bp 1.0 78.1