Hotspot

ycf15-trnL-CAA

IRb · 95474-95793 · 320 bp

Back to Hedera

Pi 0.00625
MarkerSeek score 43.20
Primer available yes
Label ycf15-trnL-CAA

Feature Metadata

label_name
ycf15-trnL-CAA
feature_type
igs
region
IRb
length_bp
320
variable_sites
4
pi
0.00625
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf15-trnL-CAA_p1 ACCCGACAGTACCCATTTTG AAATGGTAGACACGCGAGAC 50.0 50.0 57.8 58.0 435 bp 1.0 79.1
ycf15-trnL-CAA_p2 ACCCGACAGTACCCATTTTG TGAAATGGTAGACACGCGAG 50.0 50.0 57.8 58.0 437 bp 1.0 79.1
ycf15-trnL-CAA_p3 ACCCGACAGTACCCATTTTG GCCTTGGTGGTGAAATGGTA 50.0 50.0 57.8 58.1 447 bp 1.0 78.5
ycf15-trnL-CAA_p4 TATTGAATTCACCCGACCCG AAATGGTAGACACGCGAGAC 50.0 50.0 57.7 58.0 450 bp 1.0 78.1
ycf15-trnL-CAA_p5 TATTGAATTCACCCGACCCG TGAAATGGTAGACACGCGAG 50.0 50.0 57.7 58.0 452 bp 1.0 78.1