Hotspot

petA-psbJ

LSC · 65259-66292 · 1034 bp

Back to Hedera

Pi 0.00113
MarkerSeek score 50.10
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1034
variable_sites
2
pi
0.00113
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ATCCATTACGTGTCCAAGGC ATTCCGGATTGGGTTCATCC 50.0 50.0 58.0 57.6 1179 bp 1.0 76.9
petA-psbJ_p2 GGCCTTTTGTTCTTCTTGGC ATTCCGGATTGGGTTCATCC 50.0 50.0 57.8 57.6 1162 bp 1.0 76.0
petA-psbJ_p3 CATTACGTGTCCAAGGCCTT ATTCCGGATTGGGTTCATCC 50.0 50.0 58.2 57.6 1176 bp 1.0 75.8
petA-psbJ_p4 TTACGTGTCCAAGGCCTTTT ATTCCGGATTGGGTTCATCC 45.0 50.0 57.6 57.6 1174 bp 1.0 74.4
petA-psbJ_p5 TACGTGTCCAAGGCCTTTTG ATTCCGGATTGGGTTCATCC 50.0 50.0 58.4 57.6 1173 bp 1.0 74.0