Hotspot

ndhC-trnM-CAU

LSC · 52860-54709 · 1850 bp

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Pi 0.000541
MarkerSeek score 49.40
Primer available yes
Label ndhC-trnM-CAU

Feature Metadata

label_name
ndhC-trnM-CAU
feature_type
igs
region
LSC
length_bp
1850
variable_sites
2
pi
0.000541
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnM-CAU_p1 GGGGCTAAAACTCCGGAAAT CCCGCCGTATGAAAGCAATA 50.0 50.0 57.9 58.4 1986-1993 bp 1.0 75.9
ndhC-trnM-CAU_p2 CCTTTGCTAATCGGGGCTAA CCCGCCGTATGAAAGCAATA 50.0 50.0 57.7 58.4 1998-2005 bp 1.0 74.3
ndhC-trnM-CAU_p3 GGGGCTAAAACTCCGGAAAT CCGCCGTATGAAAGCAATAC 50.0 50.0 57.9 57.3 1985-1992 bp 1.0 73.9
ndhC-trnM-CAU_p4 GGGGCTAAAACTCCGGAAAT TTTGAACCAATGACTCCCGC 50.0 50.0 57.9 58.8 2001-2008 bp 1.0 73.2
ndhC-trnM-CAU_p5 CCTTTGCTAATCGGGGCTAA CCGCCGTATGAAAGCAATAC 50.0 50.0 57.7 57.3 1997-2004 bp 1.0 72.3