Hotspot

trnT-GGU-psbD

LSC · 33814-35190 · 1377 bp

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Pi 0.00121
MarkerSeek score 60.20
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1377
variable_sites
3
pi
0.00121
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 AACGGGCCCTTTTAACTCAG GGACCAGCCTACAAAAACGA 50.0 50.0 58.1 58.1 1548-1556 bp 1.0 78.9
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA GGACCAGCCTACAAAAACGA 50.0 50.0 57.9 58.1 1543-1551 bp 1.0 78.8
trnT-GGU-psbD_p3 AACGGGCCCTTTTAACTCAG ACAAAAACGAAACGGTCCCT 50.0 45.0 58.1 58.2 1538-1546 bp 1.0 77.8
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA ACAAAAACGAAACGGTCCCT 50.0 45.0 57.9 58.2 1533-1541 bp 1.0 77.8
trnT-GGU-psbD_p5 GGCGTAAGTCATCGGTTCAA GGACCAGCCTACAAAAACGA 50.0 50.0 58.3 58.1 1506-1514 bp 1.0 77.4